Thousands normally go to the annual Arlington Bluebell Walk but it was cancelled last year because of the pandemic.
The event has raised money for charity since it started in 1972.
Organisers have now confirmed on walk will be opening for a five-week period daily from Monday (April 12) 10am until 5pm.
Organiser John McCutchan said:
"We have worked incredibly hard to ensure that the site is safe and adheres to all Covid restrictions.
"We have thought long and hard on the details of how we could open but am positive we have got it right for our visitors.
"I am delighted to be opening on April 12 and thrilled that we can welcome the public back once again to the Bluebell Walk.
"The past year has been so turbulent, and Covid has affected every one of us in some way, but the future looks brighter as more people receive their jabs.
"It has to be a slimmed down version for this year to ensure the safety of our charity volunteers and our visitors.
"Nevertheless, we look forward to a successful period from Monday until May 19, and of course it is local charities which will benefit every day. And that is so important to me."
A pre-booking system has been introduced so that 65 visitors will be allowed every hour from 10am until 4pm.
Organisers will provide extra portable loos and ample hand sanitisers around the site as the safety of everyone is paramount.
Around 20 charities will benefit from the money raised.
They include the Children with Cancer Fund, Canine Partners, Hailsham Lions Club, local Rotary clubs, The JPK Project, Cancer Research UK and Memory Lane Eastbourne.
This year Bates Green Garden will be open to those who have booked to go on the Bluebell Walk, with a separate entrance fee.
Since January 2020, Emma Reece, who was a student under John's late wife Carolyn 20 years ago, has restored this tranquil plantsman's Garden, so for the first time in five years it will be open again to the public.
For more details, go to https://www.bluebellwalk.co.uk/